Fluorescence Kinetic Microscope FC 2000-Z (FKM)




The FC 2000-Z Fluorescence Kinetic Microscope (FKM) is designed to be the most versatile tool for laboratory research. Fluorescence Kinetic Microscope (FKM) extends the full capabilities of kinetic chlorophyll or multi-color fl uorescence imaging to the realm of individual cells and subcellular structures

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The Sensor measures: Fluorescence

The FC 2000-Z Fluorescence Kinetic Microscope (FKM) is designed to be the most versatile tool for laboratory research. The fluorescence kinetic microscope (FKM) extends the full imaging capabilities of kinetic chlorophyll or multi-color fluorescence to the domain of individual cells and subcellular structures. All conventional fluorescence parameters can be mapped with micrometer resolution so that individual chloroplasts or even large stromal thylakoid segments can be investigated. The modular configuration of the FKM is designed for kinetic fl uorescence measurements with various user-selectable emission and excitation wavelengths, as well as combining image measurements with spectrally resolved point measurements or ultra-fast (µs) fl uorescence kinetics. and absorbance.

The Fluorescence Kinetic Microscope (FKM) enables imaging measurements of two-dimensional resolved multi-color fluorescence transients induced by complex irradiance protocols. Fluorescence emission is induced by a suitable LED light source. In the standard configuration, the wavelength selection in the kinetic fluorescence microscope is the same as in other research grade fluorescence microscopes by using a white excitation light source combined with a set of excitation filters, dichroic mirrors and emitting filters. As an additional option, a light source with tunable spectra for measurement and actinic / saturating light is available. Increases the range of measurable chromophores and improves the signal-to-noise ratio. The system is designed to allow automatic switching between different excitation wavelengths even during the same measurement. Unlike conventional fluorescence microscopes, the ultra-high sensitivity of the measurement chamber combined with the measurement of modulated light allows images to be obtained at extremely low light levels that do not alter the metabolism of the cell.

The kinetic fluorescence microscope can be combined with spectral resolution measurements that are performed through the SM 9000 spectrometer synchronized with the measurement camera. Ultra-fast kinetic measurements are performed using a microscope-adapted version of the FL 3500 dual modulation fluorometer, which can again be synchronized with the measurement chamber. In this way, spectrally resolved or ultra-fast kinetic point measurements can be performed simultaneously on the same object and controlled by the same measurement protocol as kinetic image recording. All parts and functions can be controlled by FluorCam software based on individual settings.

Fluorescence parameters:
- Measured parameters: F0, FM, FV, F0 ', FM', FV ', FT
- More than 50 calculated parameters: FV / FM, FV '/ FM', PhiPSII, NPQ, qN, qP, Rfd, PAR absorptivity coefficient and many others
Light sources:
455nm, 470nm, 505nm, 570nm, 605nm, 618nm, 630nm, 735nm, white and others

Super pulse intensity:
- 4,000 µmol. m-2. s-1 (in standard version)
- 6,000 µmol. m-2. s-1 (in light enhanced version)

Actinic light intensity:
Up to 2000 µmol. m-2. s-1 (in standard version)
Up to 3,000 µmol. m-2. s-1 (in light enhanced version)

Filter wheel:
7 positions

Light regime:
Static or harmonically modulated (sine waveform)

Custom defined protocols:
Variable tense, special language, and scripts.

CCD detector wavelength range:
400 - 1000 nm

CCD format:
- 720 x 560 pixels (TOMI-1 camera)
- 1360 x 1024 pixels (TOMI-2 camera)
- 1280 x 1024 pixels (TOMI-3 camera)

Pixel size:
- 8.60 µm x 8.30 µm (TOMI-1)
- 6.45 µm x 6.45 µm (TOMI-2)
- 6.60 µm x 6.60 µm (TOMI-3)

Bit resolution A / D:
- 12 bits (TOMI-1)
- 16 bits (TOMI-2)
- 12 bits (TOMI-3)

Interface connector:
Gigabit Ethernet

Outside dimension:
472mm (width) x 479mm (depth) x 513mm (height)

Approx. 40 kilograms

Power input:
Approx. 1100 W

90 - 240 V

High sensitivity TOMI-1 or high resolution TOMI-2 or high speed TOMI-3

QA-Reoxidation Module
High-end option that allows the determination of QA reoxidation kinetics. The reoxidation of QA is recorded in the form of pump and probe, that is, using repetitive flashes and recording with variable delay after each flash.
PAR-Absorptivity and NDVI module
High-end option to measure PAR absorptivity and NDVI reflectance index. Includes 7 position filter wheel, 13 x 13 cm light panel (installed around the camera and mounted with 740nm far red LEDs + 660nm deep red LEDs) and a glass filter. The PAR measurement is based on the reflectance of plants in the near infrared region and in the range of the visible red spectrum. The NDVI is used as a vegetative index to predict photosynthetic activity.
IMPORTANT NOTES: For use only with FC 800-C / 1010, FC 800-C / 1010-GPF or FC 800-O / 1010. This module cannot be used with the multicolor plant module on FC 800-C / 1010 or FC 800-C / 1010-GPF.

Multicolored plant module
High-end option for measuring emission spectra in plants (according to Lichtenthaler and Bushmann). Includes 7-position filter wheel, UV LED light panel (installed around the camera and mounted with 4 380nm UV LEDs), four filters: BLUE (FF01-440 / 40), GREEN (FF02-520 / 28) , RED (FF01-690 / 8), FAR-RED (FF01-747 / 33) - and a special software module.
IMPORTANT NOTES: For use only in FC 800-C / 1010, FC 800-C / 1010-GPF or FC 800-O / 1010. This module cannot be used with the PAR-Absorptivity and NDVI module in the FC 800-C / 1010 or FC 800-C / 1010-GPF.

Rapid Fluorescence Induction Module (OJIP)
Electronic module for rapid fluorescence induction monitoring - OJIP curve. It includes a special electronic board and a measurement protocol. The processes are recorded in multiple light exposures, each with a variable delay after the light is turned on.

Filter wheel
The 7-position software-controlled filter wheel enables imaging in different emission bands. Filters are easily interchangeable.

Additional LED light panels
The additional LED light panel is mounted on top of the FluorCam system. It is located below the camera; the camera lens is inserted into the central opening of this panel. The panel expands the possibility of using more excitation colors for special applications. It is equipped with 4 high-power LEDs in the one-channel version or 8 high-power LEDs in the two-channel version. Available colors are: far red (740nm), dark red (660nm), ultraviolet (365 or 385nm), green (523nm), amber (590nm). Other options available upon request.
Ultraviolet panel (365 or 385 nm): Recommended use: (1) multicolored fluorescence: blue F440, green F520, red F680 and infrared F740 excited by UV (must be accompanied by the filter wheel and the high resolution CCD); (2) excitation source for wtGFP, DAPI (filter wheel and high resolution CCD required). Optical power 1.5 W.
Blue panel (470 nm): Recommended use: (1) excitation source for EGFP (must be accompanied by filter wheel and high resolution CCD); (2) studies on the function of stomata.
Green panel (530 nm): Recommended use: excitation source for YFP (must be accompanied by the filter wheel and high resolution CCD).
Far red panel (740 nm): Maximum total output: 1200 mW (4 x 300 mW). Recommended use: determination of absorbance of Fo 'and PAR (the use of a filter wheel is required).
PAR - NDVI Panel (660 nm + 740 nm): This panel is part of the PAR-Absorptivity and NDVI module.

Rack set for specimen holders
The racks serve for the firm and secure fixation of the sample holders (Petri dishes or multi-well plates) in the desired location on the shelf or bottom of the FluorCam. Sold as a set of six different holders: for 90mm, 100mm, 120mm or 140mm Petri dishes; 4 x 60mm Petri dish; and for 96-well plate.

Additional protective goggles
Radiation safety glasses that protect against excessive LED radiation, equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge.

Advanced multi-function for FluorCam software
Software upgrade that enables multiple scripting functions for automatic starting and measurement of scheduled FluorCam protocols and for automatic data storage. Advanced multi-function feature allows to define protocol start time with user-defined protocol, apply user-defined mask for feature segmentation, automatically subtract background based on mask definition, and analyze measured data . They are stored as TAR files with a date and time description. Useful for studying the circadian cycle.

Comprehensive module for automatic and programmable data management and image acquisition *
Complete software and hardware package for data acquisition and image parameter extraction and analysis. The software part offers an easy-to-use graphical interface, where complex protocols, including dark / light adaptation schemes, can be easily designed and executed based on user-defined actions. All acquired data is stored in a SQL database, processed and available for further inspection and analysis within seconds of recording.

It enables quick and easy exploration, grouping, analysis, reprocessing and export of user-defined data. The hardware part includes PC with RAID5 data matrix, router, monitor, keyboard and mouse.

Barcode reader with supporting software
Barcode reader with included FluorCam compatible software. Used for fully automatic barcode search.

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