FP 110 FluorPen Portable PAM Fluorometer

The FluorPen FP 110 is a portable, battery-powered PAM fluorometer that enables quick and accurate measurement of chlorophyll fluorescence parameters in the laboratory, greenhouse, or in the field. It can be effectively used for studying photosynthetic activity, stress detection, herbicide testing, or mutant screening. The affordable price and straight-forward two-button operation makes the FluorPen a perfect tool for research and for teaching photosynthesis.

The PAR-FluorPen FP 110 is an extended version of FluorPen FP 110 that incorporates an integrated light meter for direct digital readouts of Photosynthetically Active Radiation (PAR) in the range from 400 to 700 nm.

Measured data are sequentially stored in the internal FluorPen memory. Data transfer to a PC is via USB or Bluetooth communication. Comprehensive FluorPen 1.1 software provides data transfer routines and many additional features for data presentation in tables and graphs.

Different leaf-clips for gentle but firm sample holding are available:

• standard leaf-clip is suitable for experiments where short term dark adaptation is needed (version S);
• detachable leaf-clips are suitable for experiments where long term dark adaptation is needed (version D);
• custom optical probe is used in online FP version providing autonomous unattended measuring in lab conditions (version P);
• custom open-window leaf-clips are suitable for measurements in ambient light (version X).

FLUORPENS MEASURE

• Ft - continuous fluorescence yield in non-actinic light. Ft is equivalent to F₀ if the leaf sample is dark-adapted.
• QY - photosystem II Quantum Yield. QY is equivalent to Fv/Fm in the dark-adapted samples and to Fv'/Fm' in the light-adapted samples.
• OJIP - Chlorophyll Fluorescence Induction Kinetics. The OJIP curves enable observing major changes that occur during exposure of plants to high irradiance.
• NPQ - Non-Photochemical Quenching. The NPQ protocol is the most typically used measuring approach to quantify photochemical and nonphotochemical quenching. The measurement should be performed with a dark-adapted sample.
• Light Curve (QY) - Photosystem II Quantum Yield estimated from fluorescence that is measured sequentially in several different light levels.
• PAR - Photosynthetically Active Radiation measured as Photosynthetic Photon Flux Density (PPFD) (only in PAR-FluorPen FP 110)

SOFTWARE

• FluorPen 1.1 software (Windows 7 or higher compatible)
• Real-time and remote control functions
• Bluetooth and USB communication
• Visualization and data transfer routines to Microsoft Excel (optional)
• GPS mapping

Versions

Generally, all FluorPen / PAR-FluorPen versions measure the same pre-programmed fluorescence protocols and the single versions differ only in the type of used sample holder (S - standard, firmly attached leaf-clip; D - detachable leaf-clip; P - measuring probe; X - any custom-made sample holder).

Each FluorPen / PAR-FluorPen version is equipped with two communication modules - USB and Bluetooth - as well as with an in-built GPS module.

Below is detailed description of single FluorPen / PAR-FluorPen versions - with emphasis on recommended application for each version.

 -   FluorPen FP 110 / S and PAR-FluorPen FP 110 / S
 -   This version is equipped with a standard, firmly attached leaf-clip (see the picture below). It is mostly recommended for indoor experiments focusing on plants cultivated in pots, which can easily be moved to a dark place for dark adaptation.

  -  The PAR version enables measuring Photosynthetically Active Radiation (PAR) in the range from 400 to 700 nm.

  -  FluorPen FP 110 / D and PAR-FluorPen FP 110 / D
  -  This version is equipped with an optical head that allows use of detachable leaf-clips (see the picture below). These clips are intended for easy dark-adaptation of measured samples prior to measurement. The “D” version is highly recommended for field experiments. Users put the clips on selected leaves, wait required time of dark-adaptation and then measure one leaf after another (please note that detachable leaf-clips can damage very small leaves, for instance, juvenile Arabidopsis plants).

 -   The PAR version enables measuring Photosynthetically Active Radiation (PAR) in the range from 400 to 700 nm.

 -   FluorPen FP 110 / P and PAR-FluorPen FP 110 / P
 -   This version is equipped with a measuring probe and a thread for tripod attachment. The measuring probe ensures contactless sample measurement and measurement of light adapted samples. This version is recommended for automated long-term monitoring experiments.

 -  The PAR version enables measuring Photosynthetically Active Radiation (PAR) in the range from 400 to 700 nm.

 -   FluorPen FP 110 / X and PAR-FluorPen FP 110 / X
- The "X" version is mounted with a custom-made sample holder; The order of this version must be consulted before placing the order.
This version can also be offered with an optional PAR sensor to measure Photosynthetically Active Radiation (PAR) in the 400 to 700 nm range.

Accessories:

ACCESSORIES:

Leaf-Clips Detachable Leaf-Clips:

Supplementary detachable leaf-clips for use with the FluorPen FP 110/D and PAR-FluorPen FP 110/D. 10-piece set.

Control and Data Unit for Handheld Devices:

Small, portable notebook (type according to current availability on the market).

Mini-Tripod:

Small (table-top like) tripod is intended to be used with the FluorPen FP 110/P.
Note: in other FluorPen/PAR-FluorPen versions thread for tripod mounting is not integrated. However, it may be integrated on request.

Substitute USB Communication Cable:

Substitute USB cable allowing connection between the "pen" devices and a PC (the device is delivered with one USB cable, which is included in the device price).

Self-Adhesive Rubber Pads:

One set includes 10 solid pads (to be attached to the clip) and 10 pads with an opening (to be attached at the optical head). The self-adhesive rubber pads are only sold with the “S” version of FluorPens/PAR FluorPens.

Set of Replacement Joint Rings:

10-piece set O-rings 14,00 x 1,00 NBR 70 Sh for use with the FluorPen FP 110/D or PAR-FluorPen FP 110/D.

• Photosynthesis research
• Screening & Characterization of photosynthetic mutants
• Field studies
• Stress detection
• Agriculture & forestry
• Herbicide testing
• Education