JUNIOR-PAM and JUNIOR-PAM/W - Compact Fluorometer from WALZ
The two versions of the JUNIOR-PAM fluorometer provide visible light of different spectral composition: the BLUE version emits between 400 and 500 nm with a maximum around 445 nm. The WHITE version shows a narrow-band blue emission peaking around 445 nm plus a broad band emission ranging from 475 to 650 nm (Fig. 1).
Both versions are equipped with a red LED with maximum emission around 745 and an emission range from 675 to 800 nm.
|Type of Instrument||Chlorophyll Fluorometers|
|The instrument measures:||Chlorophyll Fluorescence|
Pulse amplitude modulated chlorophyll fluorometer (PAM) combined with the analysis of pulse saturation of fluorescence quenching is now a standard technique for evaluating plant photosynthesis. In the 1990s, the Education Walz PAM-fluorometer (PAM-200) took PAM fluorometry in university classrooms. Since then, there has been considerable progress in LED technology and PC, allowing the development of a powerful and versatile, while compact fluorometer teaching: the JUNIOR-PAM.
• The JUNIOR-PAM has the smallest and best price-performance for all PAM fluorometers size. It stands out for its simplicity and ease of operation.
• The JUNIOR-PAM is controlled by PC through USB interface: the fluorometer requires no external power supply as it is powered by the PC via the USB line.
• The same WinControl-3 software is provided to the investigation, as the MONITORING-PAM PAM fluorometer. In addition, WinControl-3 can be operated in a simplified to help teaching in the elementary grades so.
• The JUNIOR-PAM is particularly well suited for teaching and workshops, in which it is essential to gain practical experience in the availability of a greater number of instruments.
• Kinetic characterization of the interaction of NO with the S2 and S3-states of the oxygen-evolving complex of Photosystem II.
• PC software WinControl V3.30 (Windows 7, 8, 10) EXE-File (11 MB)
Features of Version 3.30: compatible with all WinControl fluorometers (WinControl-2 and Wincontrol-3), acquisition of spectral data, batch file operation.
• Sample data for PC software WinControl-3 ZIP-File (800 kB)
Also the spectral window for fluorescence detection distinguishes the two versions. The BLUE version detects fluorescence at wavelengths> 630 nm and the WHITE version at wavelengths> 650 nm (Fig. 2).
The BLUE version is the standard type of the JUNIOR-PAM and suitable for fluorescence measurements with most eukaryotic algae and higher plants. For algae absorbing poorly in the blue, as can be the case in species of the phylum cyanobacteria, the WHITE version yields significantly better fluorescence signals than the BLUE version.
Figure 1: Typical emission spectra of LEDs normalized to their maximum. Blue line, blue LED of JUNIOR-PAM. Gray line, white LED of JUNIOR-PAM / W. Dark red line, far red LED of JUNIOR-PAM or JUNIOR-PAM/W. Max, peak wavelength in nm. FWHM, full width at half maximum in nm.
Figure 2: Transmittance spectra of red filters positioned in front of the fluorescence detector. Red curve, JUNIOR-PAM. Dark red curve, JUNIOR-PAM/W.